Journal: Antioxidants

Article Title: Differential Regulatory Effects of Cannabinoids and Vitamin E Analogs on Cellular Lipid Homeostasis and Inflammation in Human Macrophages

doi: 10.3390/antiox15010119

Figure Lengend Snippet: Lipid accumulation in THP-1 macrophages. Cells were treated as indicated for 24 h (αT, αTAr, αTAn (50 μM); THC, CBD (6 μM)). ( A – D ) Staining was performed with Oil Red O, extraction with isopropanol, and absorbance was measured at OD 500 nm. An inhibitory effect was observed in THP-1 macrophages after treatment with αT, αTAr, and αTAn. ( B ) Stronger lipid accumulation was observed with THC, which was significantly inhibited with co-treatment with αTAn. ( C ) Significant inhibitory effects of αTAr were observed after THC co-treatment. ( D ). Stronger staining was observed with THC, which was non-significantly inhibited by co-treatment with CBD. ( E – H ) Staining was performed with Oil Red O in THP-1 macrophages and analyzed by light microscopy. THC induced more lipid droplets inside macrophages, and vitamin E acetate showed an inhibitory effect on lipid accumulation. 10× magnification. ( I – L ). Staining with Nile Red, extraction with isopropanol, and measurement by FACS at ex561/em610 nm. ( E ) A weak inhibitory effect was observed in THP-1 macrophages treated with αT, αTAr, and αTAn. ( F ) Stronger staining was induced by THC, which was significantly inhibited with co-treatment with αTAn. ( G ) Significant inhibitory effects were observed after αTAr and THC co-treatment, and αTAr had slightly stronger inhibitory effects than αTAn. ( H ) Stronger staining was observed with THC, which was significantly inhibited by co-treatment with CBD. (mean ± SEM, n = 6 for THC, CBD, αT, αTAr, αTAn, * p < 0.05, ** p < 0.005, *** p < 0.0005, **** p < 0.0001 relative to untreated control set to 100%; ns: non-significant).

Article Snippet: Subsequently, cells were stained with Oil Red O staining solution for 90 min, rinsed extensively with water, and fixed with 1 mL 10% formaldehyde/PBS (Thermo Scientific Chemicals, Waltham, MA, USA) for 1 h at room temperature.

Techniques: Staining, Extraction, Light Microscopy, Control

Journal: ACS Omega

Article Title: Gualou-Xiebai-Baijiu Decoction Ameliorates Atherosclerosis by Inhibiting the ARG1/ l ‑Arg/NO Pathway: A Study Based on Network Pharmacology

doi: 10.1021/acsomega.5c06829

Figure Lengend Snippet: Validation of MR analysis results in mice. (A,B) Oil Red O staining of the full-length mouse aorta ( n = 5, mean ± standard deviation). (C–F) Serum levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in mice ( n = 5, mean ± standard deviation). (G) Serum levels of total NO in mice ( n = 5, mean ± standard deviation). (H) Relative levels of l -Arg in mouse serum ( n = 5, mean ± standard deviation). (I,J) Relative expression of ARG1 protein in the mouse aorta ( n = 3, mean ± standard deviation). (K) Hematoxylin and eosin staining of the mouse aorta.

Article Snippet: Reagents used in this study encompassed 4% paraformaldehyde universal tissue fixative (BL539A; Labgic, Beijing, China), saturated Oil Red O staining solutions (G1015; Servicebio, Wuhan, China), and hematoxylin and eosin staining solutions (G1076; Servicebio).

Techniques: Biomarker Discovery, Staining, Standard Deviation, Expressing